Wet Chemistry

Wet chemistry techniques both qualitative and quantitative analysis

Enzymes convert raw materials into valuable products. Wet chemistry is a way of understanding enzyme activity, quality and stability on the different raw materials used and that they function as they should

Enzymes can be used on agri-waste side streams to create new business opportunities producing high value products thereby contributing to a decrease in the environmental footprint. For example, use of palm kernel cake to extract prebiotic mannan components or using food waste from the food processing industry such as oat and rice fiber after production of oat and rice drinks.

What I offer:

Qualitative/Semi Quantitative Analyses:​

• Using diffusion assays in agar plates containing relevant substrates for antimicrobial or enzymatic assays (eg. protease, NSP/fiber degrading enzymes such as xylanases, mannnanases)​
• Thin Layer chromatography visualising chain length of oligomers generated​
• SDS PAGE analyses​
• Mass spectrometry of metabolites​
  – You can see our work here https://doi.org/10.3390/microorganisms12050891​

Quantitative Analyses:​

• Non Starch Polysaccharide analyses with advice, full reporting and interpretation of data. You can read about the work here https://www.mdpi.com/1420-3049/27/2/563​
• Quantitative Spectrophotometric analyses measuring of a range of biomolecules after enzymatic /fermentation reactions such as proteins, amino acids, carbohydrates, phytate. You can read about the work here https://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0251556​
  – https://doi.org/10.3390/microorganisms12050891​